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human sirtuin 3 elisa kit  (Cusabio)


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    Cusabio human sirtuin 3 elisa kit
    Human Sirtuin 3 Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+sirtuin+3+elisa+kit/Human+NAD-dependent+deacetylase+sirtuin-3%2C+mitochondrial(SIRT3)+ELISA+kit/pm36554777-64-88-93
    Average 93 stars, based on 4 article reviews
    human sirtuin 3 elisa kit - by Bioz Stars, 2026-09
    93/100 stars

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    Enzyme-linked Immunosorbent Assay:

    Article Title: Effects of Exercise Training on Neurotrophic Factors and Blood–Brain Barrier Permeability in Young-Old and Old-Old Women
    Article Snippet: Serum SOD activity was analyzed using a colorimetric assay with the Superoxide Dismutase Assay Kit (#706002; Cayman Chemicals, Ann Arbor, MI, USA) at 450 nm with a microplate reader. .. The serum BDNF, VEGF, S100β, MMP-9, SIRT-1, SIRT-2, and SIRT-3 levels were measured by the following commercially available enzyme-linked immunosorbent assay (ELISA) kits: Human/Mouse BDNF DuoSet ELISA kit (#DY248; R&D Systems, Minneapolis, MN, USA), Human VEGF DuoSet ELISA kit (#DY293B-05; R&D Systems, Minneapolis, MN, USA), Human S100B DuoSet ELISA kit (#DY1820-05; R&D Systems, Minneapolis, MN, USA), Human MMP-9 DuoSet ELISA kit (#DY911; R&D Systems, Minneapolis, MN, USA), Human Sirtuin 1 ELISA Kit (#CSB-E15058h; CUSABIO, Wuhan, China), Human Sirtuin 2 ELISA Kit (#MBS2530369; Mybiosource, San Diego, CA, USA), and Human Sirtuin 3 ELISA Kit(#CSB-EL021341HU; CUSABIO, Wuhan, China), respectively. .. During the calculations, the absorbance values of the standards and samples were read at 450 nm by using a spectrophotometer (Tecan Sunrise, TECAN GmbH, Salzburg, Austria).

    Article Title: Effects of Exercise Training on Neurotrophic Factors and Blood-Brain Barrier Permeability in Young-Old and Old-Old Women.
    Article Snippet: Serum SOD activity was analyzed using a colorimetric assay with the Superoxide Dismutase Assay Kit (#706002; Cayman Chemicals, Ann Arbor, MI, USA) at 450 nm with a microplate reader. .. The serum BDNF, VEGF, S100β, MMP-9, SIRT-1, SIRT-2, and SIRT-3 levels were measured by the following commercially available enzyme-linked immunosorbent assay (ELISA) kits: Human/Mouse BDNF DuoSet ELISA kit (#DY248; R&D Systems, Minneapolis, MN, USA), Human VEGF DuoSet ELISA kit (#DY293B-05; R&D Systems, Minneapolis, MN, USA), Human S100B DuoSet ELISA kit (#DY1820-05; R&D Systems, Minneapolis, MN, USA), Human MMP-9 DuoSet ELISA kit (#DY911; R&D Systems, Minneapolis, MN, USA), Human Sirtuin 1 ELISA Kit (#CSB-E15058h; CUSABIO, Wuhan, China), Human Sirtuin 2 ELISA Kit (#MBS2530369; Mybiosource, San Diego, CA, USA), and Human Sirtuin 3 ELISA Kit(#CSB-EL021341HU; CUSABIO, Wuhan, China), respectively. ..



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    Figure 1. Characteristics of changes in serum levels of <t>Sirt3</t> in patients with ICU. (a) Serum Sirt3 levels were measured in sepsis group, septic shock group, and ICU control group. (b) Serum Sirt3 levels on day1, day3, day5, and day7 in patients diagnosed with sepsis on day 1. (c) Serum Sirt3 levels on day1, day3, day5, and day7 in patients diagnosed with septic shock on day 1. (d) Serum Sirt3 levels were measured in sepsis group and septic shock group after re-diagnosed on D3 and D5. P < 0.05 were considered statistically significant. *denotes P < 0.05, **denotes P < 0.01, ****denotes P < 0.0001, “ns” means no significance.
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    Cusabio sirt3
    Fig. 5. Transplantation of <t>SIRT3-overexpressing</t> AFSCs attenuated glucose meta bolic abnormalities in db/ db mice. Body weights (A) were measured, and fasting blood glucose (B), serum insulin (C), C-peptide (D), HbA1c (E) and glucagon (F) levels were determined. G. Systolic blood pressure was measured. H-J. The serum concentrations of IL6, MCP1 and TNFα were detected via ELISA assays. *P<0.05 vs WT mice, #P<0.05 vs the control group (db/db mice), @ P<0.05 vs Ad-ctrl + db/ db mice.
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    Figure 1. Characteristics of changes in serum levels of Sirt3 in patients with ICU. (a) Serum Sirt3 levels were measured in sepsis group, septic shock group, and ICU control group. (b) Serum Sirt3 levels on day1, day3, day5, and day7 in patients diagnosed with sepsis on day 1. (c) Serum Sirt3 levels on day1, day3, day5, and day7 in patients diagnosed with septic shock on day 1. (d) Serum Sirt3 levels were measured in sepsis group and septic shock group after re-diagnosed on D3 and D5. P < 0.05 were considered statistically significant. *denotes P < 0.05, **denotes P < 0.01, ****denotes P < 0.0001, “ns” means no significance.

    Journal: Scientific reports

    Article Title: Mitochondrial Sirt3 serves as a biomarker for sepsis diagnosis and mortality prediction.

    doi: 10.1038/s41598-022-14365-w

    Figure Lengend Snippet: Figure 1. Characteristics of changes in serum levels of Sirt3 in patients with ICU. (a) Serum Sirt3 levels were measured in sepsis group, septic shock group, and ICU control group. (b) Serum Sirt3 levels on day1, day3, day5, and day7 in patients diagnosed with sepsis on day 1. (c) Serum Sirt3 levels on day1, day3, day5, and day7 in patients diagnosed with septic shock on day 1. (d) Serum Sirt3 levels were measured in sepsis group and septic shock group after re-diagnosed on D3 and D5. P < 0.05 were considered statistically significant. *denotes P < 0.05, **denotes P < 0.01, ****denotes P < 0.0001, “ns” means no significance.

    Article Snippet: After defrosting the frozen serum, we used the human Sirt3 ELISA kit (Cusabio Biotech Co, Ltd, Wuhan, China, Catalog # CSB-EL021341HU) to detect the concentration of Sirt3 in the serum.

    Techniques: Control

    Figure 2. Correlation between Sirt3 and clinical indicators in ICU patients. (a) Correlation between serum Sirt3 and SOFA score in sepsis group, septic shock group, and ICU control group. (b) Correlation between serum Sirt3 and lactate in sepsis group, septic shock group, and ICU control group. (c) Correlation between serum Sirt3 and ScvO2 in sepsis group and septic shock group. (d) Correlation between serum Sirt3 and PCT in sepsis group and septic shock group. P < 0.05 were considered statistically significant. SOFA Sequential Organ Failure Assessment Score, ScvO2 Central venous oxygen saturation, PCT procalcitonin.

    Journal: Scientific reports

    Article Title: Mitochondrial Sirt3 serves as a biomarker for sepsis diagnosis and mortality prediction.

    doi: 10.1038/s41598-022-14365-w

    Figure Lengend Snippet: Figure 2. Correlation between Sirt3 and clinical indicators in ICU patients. (a) Correlation between serum Sirt3 and SOFA score in sepsis group, septic shock group, and ICU control group. (b) Correlation between serum Sirt3 and lactate in sepsis group, septic shock group, and ICU control group. (c) Correlation between serum Sirt3 and ScvO2 in sepsis group and septic shock group. (d) Correlation between serum Sirt3 and PCT in sepsis group and septic shock group. P < 0.05 were considered statistically significant. SOFA Sequential Organ Failure Assessment Score, ScvO2 Central venous oxygen saturation, PCT procalcitonin.

    Article Snippet: After defrosting the frozen serum, we used the human Sirt3 ELISA kit (Cusabio Biotech Co, Ltd, Wuhan, China, Catalog # CSB-EL021341HU) to detect the concentration of Sirt3 in the serum.

    Techniques: Control

    Figure 3. The ROC curves of indicators for the diagnosis of sepsis and the early detection of sepsis from septic shock. (a) The ROC curves of indicators for the diagnosis of sepsis (PCT, Sirt3, SOFA). (b) The ROC curves of indicators for the early detection of septic shock (lactate, Scvo2, Sirt3, lactate + Sirt3, Lactate + ScvO2, P(V-A)CO2). Calculate the predicted values for lactate + Sirt3 and lactate + ScvO2 by binary logistic regression and then calculate the ROC curve. SOFA Sequential Organ Failure Assessment Score, ScvO2 Central venous oxygen saturation, PCT procalcitonin, P(V-A)CO2 central venous-to-arterial blood carbon dioxide partial pressure.

    Journal: Scientific reports

    Article Title: Mitochondrial Sirt3 serves as a biomarker for sepsis diagnosis and mortality prediction.

    doi: 10.1038/s41598-022-14365-w

    Figure Lengend Snippet: Figure 3. The ROC curves of indicators for the diagnosis of sepsis and the early detection of sepsis from septic shock. (a) The ROC curves of indicators for the diagnosis of sepsis (PCT, Sirt3, SOFA). (b) The ROC curves of indicators for the early detection of septic shock (lactate, Scvo2, Sirt3, lactate + Sirt3, Lactate + ScvO2, P(V-A)CO2). Calculate the predicted values for lactate + Sirt3 and lactate + ScvO2 by binary logistic regression and then calculate the ROC curve. SOFA Sequential Organ Failure Assessment Score, ScvO2 Central venous oxygen saturation, PCT procalcitonin, P(V-A)CO2 central venous-to-arterial blood carbon dioxide partial pressure.

    Article Snippet: After defrosting the frozen serum, we used the human Sirt3 ELISA kit (Cusabio Biotech Co, Ltd, Wuhan, China, Catalog # CSB-EL021341HU) to detect the concentration of Sirt3 in the serum.

    Techniques: Biomarker Discovery

    Figure 4. The ROC curves of predicting for 28-day survival and Kaplan–Meier estimator analysis of Sirt3 for 28 day survival. (a) The ROC curves of Sirt3 for the predicting 28-day mortality in patients with sepsis. P < 0.05 were considered statistically significant (b) Kaplan–Meier estimator analysis of Sirt3 for 28-day survival. The cut off value of Sirt3 for the estimated 28-day mortality of patients with sepsis was 10.07 pg/ml.

    Journal: Scientific reports

    Article Title: Mitochondrial Sirt3 serves as a biomarker for sepsis diagnosis and mortality prediction.

    doi: 10.1038/s41598-022-14365-w

    Figure Lengend Snippet: Figure 4. The ROC curves of predicting for 28-day survival and Kaplan–Meier estimator analysis of Sirt3 for 28 day survival. (a) The ROC curves of Sirt3 for the predicting 28-day mortality in patients with sepsis. P < 0.05 were considered statistically significant (b) Kaplan–Meier estimator analysis of Sirt3 for 28-day survival. The cut off value of Sirt3 for the estimated 28-day mortality of patients with sepsis was 10.07 pg/ml.

    Article Snippet: After defrosting the frozen serum, we used the human Sirt3 ELISA kit (Cusabio Biotech Co, Ltd, Wuhan, China, Catalog # CSB-EL021341HU) to detect the concentration of Sirt3 in the serum.

    Techniques:

    Fig. 5. Transplantation of SIRT3-overexpressing AFSCs attenuated glucose meta bolic abnormalities in db/ db mice. Body weights (A) were measured, and fasting blood glucose (B), serum insulin (C), C-peptide (D), HbA1c (E) and glucagon (F) levels were determined. G. Systolic blood pressure was measured. H-J. The serum concentrations of IL6, MCP1 and TNFα were detected via ELISA assays. *P<0.05 vs WT mice, #P<0.05 vs the control group (db/db mice), @ P<0.05 vs Ad-ctrl + db/ db mice.

    Journal: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology

    Article Title: SIRT3 Facilitates Amniotic Fluid Stem Cells to Repair Diabetic Nephropathy Through Protecting Mitochondrial Homeostasis by Modulation of Mitophagy.

    doi: 10.1159/000489194

    Figure Lengend Snippet: Fig. 5. Transplantation of SIRT3-overexpressing AFSCs attenuated glucose meta bolic abnormalities in db/ db mice. Body weights (A) were measured, and fasting blood glucose (B), serum insulin (C), C-peptide (D), HbA1c (E) and glucagon (F) levels were determined. G. Systolic blood pressure was measured. H-J. The serum concentrations of IL6, MCP1 and TNFα were detected via ELISA assays. *P<0.05 vs WT mice, #P<0.05 vs the control group (db/db mice), @ P<0.05 vs Ad-ctrl + db/ db mice.

    Article Snippet: Published by S. Karger AG, Basel www.karger.com/cpb Feng et al.: SIRT3 Regulates Diabetic Nephropathy via AFSCs ELISA kits obtained from Cusabio Technology (Wuhan, China).

    Techniques: Transplantation Assay, Enzyme-linked Immunosorbent Assay, Control